This is a knockout-validated antibody summary, based on the publication "NIK regulates MT1-MMP activity and promotes glioma cell invasion independently of the canonical NF-κB pathway", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. From Figure 1 in [1]. Western blot of lysates of wild-type and NIK knockout BT25 glioma cells probed with anti-NIK antibody. Actin serves as a loading control.

Rabbit polyclonal

Company: Cell Signaling Technology

Antibody: NIK

Catalog number: 4994

Summary: Rabbit polyclonal antibody raised against a synthetic peptide corresponding to residues adjacent to Gly659 of human NIK.

Supplier recommended for WB.

Peptide affinity-purified.

Reacts with human and mouse NIK (predicted to react with rat, monkey, cow and horse based on sequence identity).

Western blot

Lysates of wild-type and NIK knockout BT25 glioma cells.

Anti-NIK antibody.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.