This is a knockout-validated antibody summary, based on the publication "NHERF1/EBP50 Suppresses Wnt-β-Catenin Pathway-Driven Intestinal Neoplasia", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunohistochemistry with NHERF1 antibody and DAPI for highlighting nuclei in NHERF1−/− and NHERF1+/+ mice shows high NHERF1 apical plasma membrane expression in intestinal epithelium (yellow arrowhead) and in the mesothelial lining of the serosa (arrow). From [1].

Rabbit polyclonal IgG

Company: ThermoFisher Scientific

Antibody: NHERF1

Catalog number: PA1-090

Summary: Rabbit polyclonal IgG against recombinant human EBP50. Reacts with human and mouse. Suitable for western blot, immunoprecipitation, immunocytochemistry, immunofluorescence, immunohistochemistry (paraffin), and flow cytometry. Published to react also with dog and rat.

Immunohistochemistry

Intestine from NHERF1−/− and NHERF1+/+ mice. Tissues were embedded in paraffin for sectioning.

20% to 50% donkey serum in PBS for 30 minutes.

1:200 dilution overnight in a humidified chamber at 4°C.

1:500 dilution Alexa Fluor 488 donkey anti-rabbit IgG (Molecular Probes/Invitrogen) in PBS for 45 minutes.

Zeiss 510 confocal microscope (Carl Zeiss MicroImaging, Thronwood, NY) using 40×/1.30 objective with oil immersion.