This is a knockout-validated antibody summary, based on the publication "Transcriptional regulation of ependymal cell maturation within the postnatal brain", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Coronal sections of wild-type (A-C) and Nfix−/− (D-F) brains at P15 showing expression of vimentin and NFIX. In wild-type mice, vimentin labelled cells lining the lateral ventricles (LV) clearly expressed NFIX (arrowheads in A-C). In Nfix−/− mice, however, NFIX expression was absent in ependymal cells (arrows in D and F). Scale bar (in A): 30 μm. From [1].

Mouse monoclonal IgG2aκ

Company: Sigma-Aldrich

Antibody: NFIX

Catalog number: SAB1401263

Summary: Mouse monoclonal IgG2aκ against NFIX (291 a.a. ~ 390 a.a) partial recombinant protein. Reacts with human by western blot and ELISA.

Immunohistochemistry

Wild-type and Nfix−/− mouse brains.

2% normal serum and 0.2% Triton-X-100 for 2 h.

1/200 dilution in blocking solution overnight at 4 °C.

1/250 dilution donkey anti-mouse Alexa Fluor 647 (Invitrogen) in blocking solution overnight at 4 °C.

Confocal images were acquired as consecutive 1 μm optical sections spanning a 10 μm thick Z-stack on a Zeiss inverted Axio-Observer fitted with a W1 Yokogawa spinning disk module and Hamamatsu Flash4.0 sCMOS camera and Slidebook software (3i).

The same clone (3D2) is sold as Abnova H00004784-M08; Sigma-Aldrich SAB1401263.