This is a knockout-validated antibody summary, based on the publication "NFAT2 Regulates Generation of Innate-Like CD8+ T Lymphocytes and CD8+ T Lymphocytes Responses", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of NFAT2 transcription factor in CD8+ T cells’ total protein lysates from WT and NFAT2fl/flCD4-Cre mice. GAPDH was used as loading control. From [1].

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: NFAT2

Catalog number: sc-7294

Summary: Mouse monoclonal IgG1 (kappa light chain) against amino acids 197-304 of human NFATc1. Recommended for detection of NFATc1 of human, mouse and rat origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and flow cytometry.

Western blot

CD8+ T cells total protein lysates from WT and NFAT2fl/flCD4-Cre mice. Cells were lysed in buffer containing 40 mM Tris pH 7.5, 60 mM sodium pyrophosphate, 10 mM EDTA, and 5% SDS, followed by incubation at 100°C for 15 min.

ECL Western Blotting Detection Kit (GE Healthcare).

The same clone (7A6) is sold as BioLegend 649601, 649602, 649603, 649605, 649606; Novus Biologicals NB300-620; OriGene TA328056; Santa Cruz Biotechnology sc-7294, sc-7294 AC, sc-7294 X; Enzo Life Sciences ALX-804-022-R100; Abcam ab2796; Invitrogen MA3-024; BD Biosciences 556602; Developmental Studies Hybridoma Bank 7A6.