This is a knockout-validated antibody summary, based on the publication "A critical role for NF2 and the Hippo pathway in branching morphogenesis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunohistochemistry of NF2 in the developing kidney at E12.5, E14.5, E17.5 and in adult nephrons (P90). Counterstaining with E-CADHERIN marks the ureteric bud and early nephrons. Specificity of the NF2 apical staining was confirmed using Hoxb7:Cretg/+ (Nf2UB-/-) and Six2:CreTGC/+ (Nf2CM-/-) conditional deletion of Nf2. Pink arrows point to ureteric bud tips, yellow arrowheads to the ureter/CD, blue arrowheads to early nephrons and white arrows to proximal tubules. From [1].

Rabbit polyclonal

Company: Sigma

Antibody: NF2

Catalog number: HPA003097

Summary: Rabbit polyclonal against Merlin (NF2) recombinant protein epitope. Reacts with mouse, rat, and human. Suitable for immunoblotting, immunofluorescence and immunohistochemistry.

Immunohistochemistry

IHC: Mouse kidneys from control, Hoxb7:Cretg/+ (Nf2UB-/-) and Six2:CreTGC/+ (Nf2CM-/-) conditional deletion of Nf2. Tissues were fixed in 4% paraformaldehyde and embedded in paraffin for sectioning.

IHC: 3% BSA, 10% goat serum, 0.1% Tween20 in PBS at room temperature.

WB: 1:2,000 dilution.

IHC: 1:300 dilution in 3% BSA, 3% goat serum and 0.1% Tween20 in PBS.

IHC: conjugated secondary antibodies (Jackson Laboratories).

IHC: Nikon C1 plus Digital Eclipse confocal microscope.

Western is not the best example of validation, since protein levels in KO are comparable with WT due to deletion in only one type of cells.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.