This is a knockout-validated antibody summary, based on the publications "Genome-scale CRISPR-Cas9 knockout screening in human cells", as cited below [1] and "Integrative oncogene-dependency mapping identifies RIT1 vulnerabilities and synergies in lung cancer" for western blot knockout validation (figure s5h, s5k) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Abcam
Antibody: NF2
Catalog number: ab109244
Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to the C-terminus of human NF2/Merlin. Reacts with human, mouse, rat. Suitable for western blot and immunoprecipitation.
Western blot
Control and NF2-KO (lentiCRISPR-infected) A375 cells. Cells were lysed in Cell Lysis Buffer (Cell Signaling 9803) with protease inhibitors (Sigma P8340).
1:1,000 dilution.
Membranes were developed by SuperSignal West Femto ECL (Thermo/Pierce) and imaged using BioRad ChemiDoc MP imaging system.
Please see Figure 4d in the article [1].
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