This is a knockout-validated antibody summary, based on the publication "Genome-scale CRISPR-Cas9 knockout screening in human cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit polyclonal IgG
Company: Abcam
Antibody: NF1
Catalog number: ab17963
Summary: Rabbit polyclonal IgG against an epitope mapping to a region between residue 2760 and the C terminus (residue 2818) of human Neurofibromin 1. Reacts with human, mouse, and zebrafish. Suitable for western blot and immunoprecipitation. Predicted to work with rat, rabbit, horse, cow, and monkey.
Western blot
Control and NF1-KO (lentiCRISPR-infected) A375 cells. Cells were lysed in Cell Lysis Buffer (Cell Signaling 9803) with protease inhibitors (Sigma P8340).
1:1,000 dilution.
Membranes were developed by SuperSignal West Femto ECL (Thermo/Pierce) and imaged using BioRad ChemiDoc MP imaging system.
Please see Figure S10a in the article [1].
If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.
- If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.