This is a knockout-validated antibody summary, based on the publication "Control of apico-basal epithelial polarity by the microtubule minus-end-binding protein CAMSAP3 and spectraplakin ACF7", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG1
Company: Sigma
Antibody: NEZHA
Catalog number: SAB4200415
Summary: Mouse monoclonal IgG1 against a synthetic peptide corresponding to a sequence at the C-terminal region of human NEZHA (CAMSAP3). Reacts with human and canine by western blot, immunofluorescence and immunoprecipitation.
Western blot
Control, and of CAMSAP2- and CAMSAP3-knockout Caco2 cell extracts.
1:1,000 dilution.
1:15,000 dilution IRDye-800CW- or IRDye-680LT-conjugated goat antibody against mouse IgG (#P/N 925-32210 or #P/N 925-68020) from Li-Cor Biosciences.
Western blot analysis of control, and of CAMSAP2- and CAMSAP3-knockout Caco-2 cell extracts. Please see Figure 4b in the article [1].
CAMSAP2 Rabbit 1:1000 Proteintech #17880-1-AP is also KO validated (Fig 4b, WB ) and CAMSAP2 Rabbit 1:300 Novus #NBP1-21402 is also KO validated (Fig 4a, IF).
- Noordstra I, Liu Q, Nijenhuis W, Hua S, Jiang K, Baars M, et al. Control of apico-basal epithelial polarity by the microtubule minus-end-binding protein CAMSAP3 and spectraplakin ACF7. J Cell Sci. 2016;129:4278-4288 pubmed
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