This is a knockout-validated antibody summary, based on the publication "A flexible, multilayered protein scaffold maintains the slit in between glomerular podocytes", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Sheep polyclonal IgG

Company: R&D

Antibody: NEPH2

Catalog number: AF4910

Summary: Sheep polyclonal IgG against mouse myeloma cell line NS0-derived recombinant human Kirrel3/NEPH2 Tyr33-Ala535. Reacts with human, mouse and rat by western blot.

Western blot

Brain lysates from WT and Neph2-KO ice. Tissues were glass-glass-homogenized in RIPA buffer containing: 50mM Tris/HCl pH 7,5, 1 mM EGTA, 1 mM EDTA, 1% (w/v) Triton X-100, 0,1 % (w/v) SDS, 50 mM NaF, 150 mM NaCl, 0,5% (w/v) Na-Deoxycholate, 0,1 %(v/v) 2-mercaptoethanol, 1mM Na-Orthovanadate, Roche Ultra complete proteinase inhibitor cocktail and Roche Phospho-STOPP as indicated by the manufacturer (Roche, Mannheim, Germany) and ddH2O ad final volume; 15μl lysis buffer per 1 mg of tissue were used).

HRP-coupled secondary antibodies.

ECL in combination with a conventional x-ray system (films: Fuji, Tokio, Japan; developer: AGFA, Mortsel, Belgium).

Please see Supplemental Figure 2c in the article [1].