This is a knockout-validated antibody summary, based on the publication "NEDD9 stabilizes focal adhesions, increases binding to the extra-cellular matrix and differentially effects 2D versus 3D cell migration", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot analysis of extracts of wild-type (WT) and NEDD9 knockout (−/−) MEFs. Blots were probed with antibodies to NEDD9 which detects the 105 kD and 115 kD phospho-forms of NEDD9. Anti-tubulin was used to confirm equal loading. From [1].

Mouse monoclonal IgG1 kappa

Company: ImmuQuest

Antibody: NEDD9

Catalog number: IQ296

Summary: Mouse monoclonal IgG1 kappa against a fusion protein corresponding to amino acids 82-398 of human HEF1. Reacts with mouse, rat, and human. Suitable for western blot, immunoprecipitation, immunocytochemistry, immunohistochemistry-frozen and flow cytometry.

Western blot

NEDD9 knockout (−/−) MEFs

The same clone ('2G9) is sold as OriGene TA319568; Santa Cruz Biotechnology sc-33659; Abnova MAB2476; ImmuQuest IQ297, IQ296; Rockland Immunochemicals 200-301-904; Invitrogen MA1-5784, MA1-5785; Cell Signaling Technology 4044S; EMD Millipore MABT404.