This is a knockout-validated antibody summary, based on the publication "Bipolar cell reduction precedes retinal ganglion neuron loss in a complex 1 knockout mouse model", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG2a (kappa light chain)
Company: Santa Cruz
Antibody: NDUFS4
Catalog number: sc-100567
Summary: Mouse monoclonal IgG2a (kappa light chain) against recombinant human NDUFS4. Recommended for detection of NDUFS4 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.
Western blot
Retinas of WT and Ndufs4-KO were dissected and homogenized in 2x cell lysis buffer (Cell Signaling).
1:1,000 dilution overnight at 4 C.
IRDye 680LT goat anti-mouse.
Odyssey LI-COR was used to scan signals.
Western-blot results using anti-Ndufs4 antibody in retinas (WT = 3,
KO = 3). Tubulin was used as loading control. Please see Figure 1a in the article [1].
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