NCS1 antibody | knockout validation | Abcam ab129166

This is a knockout-validated antibody summary, based on the publication "Calcium Sensor, NCS-1, Promotes Tumor Aggressiveness and Predicts Patient Survival", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: NCS1

Catalog number: ab129166

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues on the C terminal of human NCS1. Reacts with mouse, rat and human. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin) and flow cytometry.

Validation Method

Immunohistochemistry

Sample

Brain tissues from NCS-1 knockout mice and wild-type controls.

Blocking agent

0.3%BSA/ TBST solution for 1 h at room temperature.

Primary incubation

1:1000 dilution in BSA/TBST solution at 4 °C overnight.

Secondary incubation

Anti-rabbit poly HRP antibody Envision (DAKO, Carpinteria, CA, USA) for 1 h at room temperature.

Detection

Cy5 tyramide at room temperature for 10 min.

Figure Photomicrograph of cerebellum tissue from wild type and NCS-1 knockout mice stained for NCS-1 and DAPI reveals specificity of NCS-1 antibody. Please see Figure 1b in the article [1].
References
  1. Moore L, England A, Ehrlich B, Rimm D. Calcium Sensor, NCS-1, Promotes Tumor Aggressiveness and Predicts Patient Survival.. Mol Cancer Res. 2017;15:942-952 pubmed publisher