This is a knockout-validated antibody summary, based on the publication "The CUL5 Ubiquitin Ligase Complex Mediates Resistance to CDK9 and MCL1 Inhibitors in Lung Cancer Cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blotting validating loss of CUL5 and UBE2F protein in the three CUL5-KO clones and one UBE2F-KO clone, respectively. GAPDH, loading control. From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: NCE2/UBE2F

Catalog number: ab185234

Summary: Rabbit monoclonal IgG against a recombinant fragment within human NCE2/UBE2F (aa 1-150, N terminal). Reacts with human by western blot, immunohistochemistry (paraffin), flow cytometry and immunocytochemistry/immunofluorescence.

Western blot

Parental and UBE2F-KO LK2 cells.

Manufacturer’s recommended dilutions were used.

1:10,000 dilution donkey anti-rabbit 680 (925–68023; LI-COR) or donkey anti-rabbit 800 (925–32213; LI-COR).

Blots were imaged on an Odyssey CLx Imaging System (LI-COR).