This is a knockout-validated antibody summary, based on the publication "RRRRR", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

[enlarge]

Figure 1. Western blot analysis of CD56 from wild-type (WT) and CD56-knockout (KO) YTS (left) and NK92 (right) cell lines or primary human NK cells with actin as a loading control. From [1].

Mouse monoclonal IgG1

Company: Cell Signaling

Antibody: NCAM1 (CD56)

Catalog number: 3576

Summary: Mouse monoclonal IgG1 against a membrane preparation of a small cell lung carcinoma specimen. Reacts with human. Suitable for western blot, immunohistochemistry (paraffin), immunofluorescence/immunocytochemistry, and flow cytometry.

Western blot

5% nonfat milk in PBS 0.05% Tween-20 for 90–120 min at 4°C.

1:1,000 dilution in 5% (w/v) BSA in PBS 0.05% Tween 20 overnight at 4°C.

1:10,000 dilution IRDye 680RD goat anti-mouse IgG (Li-COR Biosciences) for 1 hr at room temperature.

Odyssey CLx imaging system (Li-COR Biosciences).

The same clone (123C3) is sold as Bio-Rad MCA2693GA, MCA2693A488, MCA2693A700, MCA2693A647, MCA2693A647T, MCA2693, MCA2693A488T, MCA2693F; Santa Cruz Biotechnology sc-7326; LifeSpan Biosciences LS-B5569, LS-B7180, LS-C188381; Abnova MAB16162, MAB16163, MAB16238; Abcam ab9272; Invitrogen MA1-06801, MA1-35249, MA5-16446, MA5-16445, 07-5603; MyBioSource MBS217370; Cell Signaling Technology 3576; Dako M7304.