This is a knockout-validated antibody summary, based on the publication "The adherens junctions control susceptibility to Staphylococcus aureus α-toxin", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal

Company: Millipore

Antibody: N-cadherin

Catalog number: 05-915

Summary: Mouse monoclonal against human N-cadherin cytoplasmic domain MBP fusion protein. Reacts with human. Suitable for western blot, immunocytochemistry, immunohistochemistry and immunoprecipitation.

Western blot

WT and N-cadherin-knockout HAP1 cells. Cell pellets were lysed with hot Laemmli SDS sample buffer containing 5% (vol/vol) β-mercaptoethanol and boiled for 10 min.

PBS buffer containing 5% (wt/vol) nonfat milk and 0.01% Tween-20 for 1 h at room temperature.

1:2,000 dilution in blocking buffer overnight at 4 °C.

1:5,000 dilution HRP-conjugated anti-mouse secondary antibodies (GeneTex) in blocking buffer for 1 h at room temperature.

West Pico and Extended Duration chemiluminescence peroxide solutions (Thermo) and film exposure.

Please see Figure S1 in the article [1].

The same clone (13A9) is sold as Bio-Rad MCA5698; BioLegend 844702, 844703; Novus Biologicals NBP1-48309, NBP1-48309AF405, NBP1-48309AF488, NBP1-48309AF647, NBP1-48309AF700, NBP1-48309APC, NBP1-48309B, NBP1-48309C, NBP1-48309F, NBP1-48309FR, NBP1-48309G, NBP1-48309H, NBP1-48309IR, NBP1-48309PCP, NBP1-48309PE, NBP1-48309R, NBP1-48309UV, NBP1-48309V, NBP1-48309V3; OriGene TA336779; Santa Cruz Biotechnology sc-59987; LifeSpan Biosciences LS-C143420, LS-C143421, LS-C143422, LS-C143423, LS-C143425, LS-C187536; Invitrogen MA1-159; EMD Millipore 05-915.