N WASP antibody | knockout validation | Abcam ab126626

This is a knockout-validated antibody summary, based on the publication "Entry by Multiple Picornaviruses Is Dependent on a Pathway That Includes TNK2, WASL, and NCK1", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

N WASP antibody | knockout validation | Abcam ab126626 figure 1
Figure 1. Western blot WASL protein expression in WASL KO and Ctrl (control) A549 cells. The red asterisk indicates WASL protein band. Antibody is not 100% specific, since it recognizes a very strong nonspecific band. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: N WASP

Catalog number: ab126626

Summary: Rabbit monoclonal IgG against a synthetic peptide within human N WASP (aa 300-450). Reacts with mouse, human and pig. Suitable for western blot, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin) and flow cytometry.

Validation Method

Western blot

Sample

Control and WASL KO A549 cells.

Blocking agent

5% skim milk.

Primary incubation

1:1,000 dilution in 5% milk overnight at 4°C.

Secondary incubation

HRP conjugated secondary antibodies were incubated in 5% milk at room temperature for 1 hr.

Detection

Chemiluminescent substrate for 5 min and then imaged using a Bio-Rad chemiluminescence imager.

References
  1. Jiang H, Leung C, Tahan S, Wang D. Entry by multiple picornaviruses is dependent on a pathway that includes TNK2, WASL, and NCK1. elife. 2019;8: pubmed publisher