N-Myc antibody | knockout validation

N-Myc antibody | knockout validation | Santa Cruz sc-53993

This is a knockout-validated antibody summary, based on the publication "Distinct molecular pathways mediate Mycn and Myc-regulated miR-17-92 microRNA action in Feingold syndrome mouse models", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblot analysis for indicated proteins upon treatment with 10% fetal bovine serum (FBS) at indicated time points. Primary mesenchymal fibroblasts from limb buds of E10.5 Mycnfl/fl embryos were transduced with adenoviruses expressing a Cre recombinase (KO) or a yellow fluorescent protein (Ctrl) to delete Mycn in vitro. From [1].

Antibody information

Mouse monoclonal IgG2a (kappa light chain)

Company: Santa Cruz

Antibody: N-Myc

Catalog number: sc-53993

Summary: Mouse monoclonal IgG2a (kappa light chain) against N-terminal recombinant human N-Myc. Recommended for detection of N-Myc of mouse, rat and human origin by western blot and immunoprecipitation.

Validation Method

Western blot

Sample

Control (Mycnfl/fl) and Mycn-KO (Cre recombinase-transduced) primary mesenchymal fibroblasts from limb buds of E10.5 Mycnfl/fl embryos.

References

Mirzamohammadi F, Kozlova A, Papaioannou G, Paltrinieri E, Ayturk U, Kobayashi T. Distinct molecular pathways mediate Mycn and Myc-regulated miR-17-92 microRNA action in Feingold syndrome mouse models. Nat Commun. 2018;9:1352 pubmed publisher

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