This is a knockout-validated antibody summary, based on the publication "The molecular basis of the genesis of basal tone in internal anal sphincter", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blots of MLCK in control and MlckSMKO IAS smooth-muscle cells collected 20 days after tamoxifen injection. β-actin was used as the protein loading control. From [1].

Mouse monoclonal IgG2b

Company: Sigma

Antibody: Myosin Light Chain Kinase

Catalog number: M7905

Summary: Mouse monoclonal IgG2b against chicken gizzard myosin light chain kinase. Reacts with avian and mammals. Suitable for western blot, immunoprecipitation and microarray.

Western blot

Control and MlckSMKO internal anal sphincter (IAS) smooth-muscle cells. IAS smooth muscle strips were homogenated in 120 μl lysis buffer (containing 20 mM Tris base, 137 mM NaCl, 2 mM EDTA, 1% NP-40, 10% glycerol, protease inhibitor cocktail (Roche), pH=8).

1:10,000 dilution.

Super Signal West Dura substrate (PIERCE) and MaxiSignal Western Solution (SUDGEN) were used.