This is a knockout-validated antibody summary, based on the publication "Feedback regulation of Notch signaling and myogenesis connected by MyoD-Dll1 axis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blotting results to show the expression levels of myogenic markers in WT and MyoGKO myoblasts. Star highlights a non-specific band. From [1].

Mouse monoclonal IgG1, kappa

Company: DSHB

Antibody: Myogenin

Catalog number: F5D

Summary: Mouse monoclonal antibody against a recombinant rat myogenin protein. Reacts with canine, feline, human, mammal, mouse, porcine, and rat. Suitable for western blot, immunofluorescence, immunohistochemistry (paraffin), immunoprecipitation (IP), Chromatin IP, and ELISA.

Western blot | Immunocytochemistry

WT and MyoG-KO mouse and human myoblasts.

WB: 5% milk at room temperature.

ICC: 3% BSA at room temperature.

WB: Diluted in 5% milk overnight at 4°C.

ICC: overnight at 4°C.

WB: 1:5,000 dilution Goat Anti-Mouse IgG (H+L)-HRP Conjugate (Bio-Rad, 170–6516).

ICC: fluorescence conjugated secondary antibodies.

WB: Western Blotting Luminol Reagent (Santa Cruz Biotechnology, sc2048).

ICC: BioTek Microscope System or Olympus FV1200 Confocal Laser Scanning Microscope.

The same clone (F5D) is sold as Novus Biologicals NBP2-44328; OriGene TA327708, AM00317SU-N, AM33347PU-S, AM33347PU-T; Santa Cruz Biotechnology sc-12732, sc-12732 AC, sc-12732 X; LifeSpan Biosciences LS-C87542, LS-C95519, LS-C202887, LS-C312116, LS-C348538; Abcam ab1835; Invitrogen 14-5643-80, 14-5643-82, 53-5643-80, 53-5643-82, MA5-11486, 14-5643-95, 14-5643-37; MyBioSource MBS370091; Dako M3559; BD Biosciences 556358; Developmental Studies Hybridoma Bank F5D.