MyD88 antibody | knockout validation | Cell Signaling 4283

This is a knockout-validated antibody summary, based on the publications "NFATC3 promotes IRF7 transcriptional activity in plasmacytoid dendritic cells" [1] (discussed below) and article "Ebola virus glycoprotein directly triggers T lymphocyte death despite of the lack of infection" [2] (western blot, see figure 4b in the article), and "Epigenetic state determines inflammatory sensing in neuroblastoma" for western blot knockout validation (figure s13a) [3]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

MyD88 antibody | knockout validation | Cell Signaling 4283 figure 1
Figure 1. Western blot of MyD88 and β-actin (loading control) in parental and MyD88 knockout Gen2.2 cells. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: MyD88

Catalog number: 4283

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Cys233 of human MyD88 protein. Reacts with human, mouse, rat, and monkey. Suitable for western blot and immunoprecipitation.

Validation Method

Western blot

Sample

Control and MyD88 knockout Gen2.2 cells.

References
  1. Bao M, Wang Y, Liu Y, Shi P, Lu H, Sha W, et al. NFATC3 promotes IRF7 transcriptional activity in plasmacy--toid dendritic cells. J Exp Med. 2016;213:2383-2398 pubmed
  2. Iampietro M, Younan P, Nishida A, Dutta M, Lubaki N, Santos R, et al. Ebola virus glycoprotein directly triggers T lymphocyte death despite of the lack of infection. PLoS Pathog. 2017;13:e1006397 pubmed publisher
  3. Wolpaw A, Grossmann L, Dessau J, Dong M, Aaron B, Brafford P, et al. Epigenetic state determines inflammatory sensing in neuroblastoma. Proc Natl Acad Sci U S A. 2022;119: pubmed publisher