This is a knockout-validated antibody summary, based on the publication "Msi RNA-binding proteins control reserve intestinal stem cell quiescence", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Msi1 and Msi2 staining in normal intestinal crypts under basal conditions in control and Msi-DKO mice. Bar, 50 µM. From [1].

Rat monoclonal IgG2a

Company: MBL International

Antibody: Musashi-1

Catalog number: D270-3

Summary: Rat monoclonal IgG2a kappa against recombinant mouse Musashi-1 (190-362 aa). Reacts with mouse by western blot and immunohistochemistry.

Immunohistochemistry

Intestinal epithelium from control and Msi1/2flx/flx::Villin-CreERT2 (Msi-DKO) mice. Tissues were fixed in 4% PFA overnight at 4°C, washed in PBS, and moved to 70% ethanol before paraffin embedding and sectioning.

1:200 dilution.

Cy2-, Cy3-, and Cy5-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, Inc.)

Leica Biosystems SP5 (LAS AF software) and SP8 confocal microscopes (LAS X software) were used for fluorescence microscopy equipped with 10× dry, 20× dry, 40× water, and 63× oil objectives.

Musashi-2 antibody (1:200, NBP1-42029; Novus Biologicals) is also KO validated in the same Figure.