Mus81 antibody | knockout validation | Abcam ab14387

This is a knockout-validated antibody summary, based on the publications "The DNA repair endonuclease Mus81 facilitates fast DNA replication in the absence of exogenous damage", as cited below [1], and "Bi-allelic MCM10 variants associated with immune dysfunction and cardiomyopathy cause telomere shortening" for western blot knockout validation (figure 8a) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Mouse monoclonal IgG2a

Company: Abcam

Antibody: Mus81

Catalog number: ab14387

Summary: Mouse monoclonal IgG2a against Mus81 protein. Reacts with human by western blot, immunoprecipitation, immunocytochemistry/immunofluorescence and flow cytometry.

Validation Method

Western blot

Sample

Wildtype, Mus81−/− and GFP-Mus81-complemented HCT116 cells. Whole cell lysates were prepared with 1x SDS loading buffer.

Primary incubation

1:2,000 dilution.

Note

Western-blot of Mus81 in wildtype, Mus81−/− HCT116 cells and doxycycline-inducible GFP-Mus81-complemented stable cell lines. Please see Figure 5b in the article [1].

Clone note

The same clone (2G10/3) is sold as Santa Cruz Biotechnology sc-47692; LifeSpan Biosciences LS-C82284; Abnova MAB2474; ImmuQuest IQ285.

References
  1. Fu H, Martin M, Regairaz M, Huang L, You Y, Lin C, et al. The DNA repair endonuclease Mus81 facilitates fast DNA replication in the absence of exogenous damage. Nat Commun. 2015;6:6746 pubmed publisher
  2. Baxley R, Leung W, Schmit M, Matson J, Yin L, Oram M, et al. Bi-allelic MCM10 variants associated with immune dysfunction and cardiomyopathy cause telomere shortening. Nat Commun. 2021;12:1626 pubmed publisher