This is a knockout-validated antibody summary, based on the publication "Molecular Implications of MUC5AC-CD44 Axis in Colorectal Cancer Progression and Chemoresistance", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG1
Company: Millipore
Antibody: Mucin MUC5AC
Catalog number: MAB2011
Summary: Mouse monoclonal IgG1 against a synthetic peptide of the MUC5AC tandem repeat. Reacts with human by western blot, immunoprecipitation, immunocytochemistry, immunohistochemistry (paraffin) and ELISA.

Western blot
WT and MUC5AC-KO HCT-8 and LS174T cell lines. Total protein lysates were prepared in radio immunoprecipitation assay (RIPA) lysis buffer containing 50 mM Tris-HCl, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate along with protease inhibitor cocktail (Roche Diagnostics, Mannheim, Germany).
5% non-fat dry milk having phosphate-buffered saline with 0.1% Tween 20 (PBST) for 1 h.
At 4 °C overnight.
For 1 h at room temperature.
Chemiluminescence reagent (GE Healthcare Bio-Sciences, PA, USA).
The same clone (CLH2) is sold as Santa Cruz Biotechnology sc-33667; LifeSpan Biosciences LS-B2434, LS-C152909, LS-C312111, LS-C348684; Abnova MAB13373, MAB13374; EMD Millipore MAB2011.
- If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.
- product
- Abnova MUC5AC monoclonal antibody, clone CLH2
- Abnova MUC5AC monoclonal antibody, clone CLH2
- EMD Millipore Mucin MUC5AC Antibody, clone CLH2
- LifeSpan Biosciences IHC-plus⢠MUC5AC Antibody (clone CLH2) LS-B2434
- LifeSpan Biosciences MUC5AC Antibody (clone CLH2) LS-C152909
- LifeSpan Biosciences MUC5AC Antibody (clone CLH2) LS-C312111
- LifeSpan Biosciences MUC5AC Antibody (clone CLH2, Ready-to-Use) LS-C348684
- Santa Cruz Biotechnology Mucin 5AC (CLH2) Antibody