This is a knockout-validated antibody summary, based on the publication "Molecular Implications of MUC5AC-CD44 Axis in Colorectal Cancer Progression and Chemoresistance", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblot showing deletion of MUC5AC in clones of CRC HCT-8 cell line. From [1].

Mouse monoclonal IgG1

Company: Millipore

Antibody: Mucin MUC5AC

Catalog number: MAB2011

Summary: Mouse monoclonal IgG1 against a synthetic peptide of the MUC5AC tandem repeat. Reacts with human by western blot, immunoprecipitation, immunocytochemistry, immunohistochemistry (paraffin) and ELISA.

Figure 2. Immunoblot showing deletion of MUC5AC in clones of CRC LS174T cell lines. From [1].

Western blot

WT and MUC5AC-KO HCT-8 and LS174T cell lines. Total protein lysates were prepared in radio immunoprecipitation assay (RIPA) lysis buffer containing 50 mM Tris-HCl, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate along with protease inhibitor cocktail (Roche Diagnostics, Mannheim, Germany).

5% non-fat dry milk having phosphate-buffered saline with 0.1% Tween 20 (PBST) for 1 h.

At 4 °C overnight.

For 1 h at room temperature.

Chemiluminescence reagent (GE Healthcare Bio-Sciences, PA, USA).

The same clone (CLH2) is sold as Santa Cruz Biotechnology sc-33667; LifeSpan Biosciences LS-B2434, LS-C152909, LS-C312111, LS-C348684; Abnova MAB13373, MAB13374; EMD Millipore MAB2011.