This is a knockout-validated antibody summary, based on the publication "Functional role of Mst1/Mst2 in embryonic stem cell differentiation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunoblotting analysis of the expression of Mst1 in wild type ES cells and two independent Mst1/Mst2 double knockout (Mst-/-1 and Mst-/-2) ES cell lines. Gapdh1 was used as a loading control. From [1].

Rabbit polyclonal

Company: Cell Signaling

Antibody: Mst1

Catalog number: 3682

Summary: Rabbit polyclonal against a synthetic peptide corresponding to the amino-terminal region of human MST1 protein. Reacts with human, mouse, rat, monkey and bovine. Suitable for western blot and immunoprecipitation.

Western blot

Wild type and Mst1/Mst2 double knockout ES cells. RIPA buffer (150 mM NaCl, 10 mM Tris, pH 7.2, 0.1% SDS, 1.0% Triton X-100, 1.0% deoxycholate, 5 mM EDTA, and protease inhibitors) was used to lyse the cells.

5% nonfat dry milk (BD company) in TBST+0.1% Tween.

1/1,000 in TBST+0.1% Tween overnight at 4°C.

ECL detection reagents (Abcam).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.