This is a knockout-validated antibody summary, based on the publication "Mesalazine and thymoquinone attenuate intestinal tumour development in Msh2(loxP/loxP) Villin-Cre mice", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. C) Msh2 expression is depleted in intestinal epithelial cells but not in pancreatic tissue and lamina propria (arrow) from Msh2loxP/loxP Villin-Cre mice (left). (D) Control mice (wildtype) express Msh2 mainly in the crypts. From [1].

Rabbit polyclonal IgG

Company: Bethyl

Antibody: Msh2

Catalog number: IHC-00082

Summary: Rabbit polyclonal IgG against an epitope of human Msh2 protein between 875 and C-term. Reacts with human and mouse. Suitable for immunohistochemistry (paraffin) and immunofluorescence.

Immunohistochemistry

Intestine from 4-week-old to 6-week-old female and male Msh2loxP/loxP Villin-Cre mice. Tissue was fixed in neutral buffered formalin for 24 h and paraffin-embedded.

10% goat serum or 2% horse serum and 3% BSA.

1:250 dilution.

Avidin-biotin-horseradish peroxidase complex (VECTASTAIN Elite ABC Kit, Vector Laboratories).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.