Mpeg1 antibody | knockout validation | Abcam ab25146
DOI
//dx.doi.org/10.13070/ko.en.6.1675
Date
2016-10-04

This is a knockout-validated antibody summary, based on the publication "Perforin-2 is essential for intracellular defense of parenchymal cells and phagocytes against pathogenic bacteria", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mpeg1  antibody | knockout validation | Abcam ab25146 figure 1
Figure 1. Western blot for perforin-2 in murine peritoneal macrophages (upper panel) or neutrophils (lower panel) isolated from perforin-2 wild-type (wt), perforin-2 heterozygous (het), or perforin-2 homozygous knockout (ko) mice. Beta-actin was used as loading control. From [1].
Antibody information

Antibody: Mpeg1

Catalog number: ab25146

Summary: Rabbit polyclonal against recombinant full length Mpeg1 protein. It detects mouse and rat proteins in western blot.

Company: Abcam

Validation Method

Western blot

Sample

Murine peritoneal macrophages (upper) or neutrophils (lower) were isolated from perforin-2 wild-type (wt), perforin-2 heterozygous (het), or perforin-2 homozygous knockout (ko) mice.

Disclaimer

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.

References
  1. McCormack R, de Armas L, Shiratsuchi M, Fiorentino D, Olsson M, Lichtenheld M, et al. Perforin-2 is essential for intracellular defense of parenchymal cells and phagocytes against pathogenic bacteria. elife. 2015;4: pubmed publisher