This is a knockout-validated antibody summary, based on the publication "Interaction between Plasmodium Glycosylphosphatidylinositol and the Host Protein Moesin Has No Implication in Malaria Pathology", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot analysis of moesin protein expression by WT and moesin gene knockout mice. Moesin detection with anti-moesin antibody clone EP1863Y indicates absence of moesin in moesin-deficient mice; THP1 cells were used as positive control for moesin detection, GAPDH detection served as loading control. From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: Moesin

Catalog number: ab52490

Summary: Rabbit monoclonal IgG against a synthetic peptide within human moesin aa 450-550 (C terminal). Reacts with mouse, rat, and human. Suitable for western blot, immunocytochemistry/immunofluorescence, immunohistochemistry (frozen and paraffin) immunoprecipitation, and flow cytometry.

Western blot

WT and moesin gene knockout mice BMDM. Cells were lysed by resuspension in sample buffer (Laemmli 2x concentrate, Sigma-Aldrich).

Anti-rabbit antibody coupled to HRP (Jackson Immunoresearch, UK).

ECL Western blotting substrate (Pierce, Germany).