Mitofusin-2 antibody | knockout validation | Cell Signaling 9482

This is a knockout-validated antibody summary, based on the publication "Mitofusins regulate lipid metabolism to mediate the development of lung fibrosis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mitofusin-2 antibody | knockout validation | Cell Signaling 9482 figure 1
Figure 1. Representative immunoblots of AEC2 cell lysates obtained 3 weeks after tamoxifen-induced deletion, showing decreased protein levels of MFN1 or MFN2 in the respective knockout cells (n = 3 mice per group). From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Mitofusin-2

Catalog number: 9482

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Val573 of human mitofusin-2 protein. Reacts with human, mouse, rat, hamster, and monkey. Suitable for western blot, immunohistochemistry (paraffin) and immunofluorescence /immunocytochemistry.

Validation Method

Western blot

Sample

Control and MFN2-KO mice lung alveolar type 2 epithelial cell (AEC2).

Primary incubation

1:1,000 dilution.

Secondary incubation

1:5,000 dilution HRP-conjugated secondary anti-rabbit IgG (Santa Cruz sc-2004 or GeneTex 213110).

References
  1. Chung K, Hsu C, Fan L, Huang Z, Bhatia D, Chen Y, et al. Mitofusins regulate lipid metabolism to mediate the development of lung fibrosis. Nat Commun. 2019;10:3390 pubmed publisher