This is a knockout-validated antibody summary, based on the publication "SZT2 dictates GATOR control of mTORC1 signalling", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Cell Signaling
Antibody: Mios
Catalog number: 13557
Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Leu730 of human Mios protein. Reacts with human, mouse and rat. Suitable for western blot and immunoprecipitation.
Western blot
Control and MIOS-KO HEK293T cells. Cells were lysed with the CHAPS or Triton X-100 lysis buffer (40 mM HEPES, pH 7.4, 0.3% CHAPS or 1% Triton X-100, 10 mM β-glycerol phosphate, 10 mM pyrophosphate and 2.5 mM MgCl2) supplemented with the EDTA-free protease inhibitor and PhosSTOP.
Immunoblotting of the indicated proteins in lysates of control and MIOS-KO cells. Please see Figure 2f in the article [1].
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