Microcephalin-1/BRIT1 antibody | knockout validation | Cell Signaling 4120

This is a knockout-validated antibody summary, based on the publication "TALEN-based generation of a cynomolgus monkey disease model for human microcephaly", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Microcephalin-1/BRIT1 antibody | knockout validation | Cell Signaling 4120 figure 1
Figure 1. Western blot confirms that MCPH1 expression is reduced in MCPH1mt/mt monkey dermal fibroblasts (DFs) and peripheral blood mononuclear cells (PBMCs). MCPH1mt/mt, MCPH1 mutant monkey; Con, wild-type monkey. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Microcephalin-1/BRIT1

Catalog number: 4120

Summary: Rabbit monoclonal IgG a synthetic peptide corresponding to central residues of human Microcephalin-1/BRIT1. Reacts with human, mouse, rat, and monkey by western blot.

Validation Method

Western blot

Sample

Total protein extracts from dermal fibroblasts (DFs) and peripheral blood mononuclear cells (PBMCs) from WT and MCPH1 mutant monkey.

Primary incubation

1:1,000 dilution

Detection

Chemiluminescence (Amersham) was used to visualise the protein bands on X-ray films (Kodak).

References
  1. Ke Q, Li W, Lai X, Chen H, Huang L, Kang Z, et al. TALEN-based generation of a cynomolgus monkey disease model for human microcephaly. Cell Res. 2016;26:1048-61 pubmed publisher