This is a knockout-validated antibody summary, based on the publication "Mind Bomb-2 Regulates Hippocampus-dependent Memory Formation and Synaptic Plasticity", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of Mib2 protein expression in mouse hippocampi from WT and Mib2 KO littermates. Actin is used as loading control. from figure 4 [1].

Company: Abnova

Antibody: Mib2

Catalog number: H00142678-M01A

Summary: Mouse IgG2a kappa monoclonal antibody raised against a partial recombinant MIB2 peptide (AAH37542, 381 a.a. ~ 480 a.a) with a GST tag. Reacts with human protein and has been used in western blot and ELISA.

Western Blot.

Mice (male) Mib2 KO hippocampi frozen immediately in liquid nitrogen and stored at -80℃ until lysis with a lysis buffer (1.6% SDS, 10 mM TrisCl; pH 6.8). Protease inhibitor cocktail (Roche 11873580001) and protein phosphatase inhibitor cocktail (Roche 04906845001) were also added to the lysis buffer. Lysed with the QIAGEN TissueLyser LT (50 Hz, 3 min).