This is a knockout-validated antibody summary, based on the publication "MITF reprograms the extracellular matrix and focal adhesion in melanoma", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot showing the MITF band in EV-SkMel28, deltaMITF-X2, and deltaMITF-X6 cell lines. From [1].

Mouse monoclonal IgG1

Company: Abcam

Antibody: MiTF

Catalog number: ab12039

Summary: Mouse monoclonal antibody against a recombinant fragment corresponding to human MiTF (N terminal). Reacts with human. It is predicted to work with mouse, rat, and chicken. Suitable for western blot and flow cytometry.

Western blot | Immunocytochemistry

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Figure 2. Immunostaining for MITF and Vimentin in EV-SkMel28, deltaMITF-X2, and deltaMITF-X6 cell lines, scale bar (10 um). From [1].

Control and MITF-KO SkMel28 cell lines.

WB: 5% bovine serum albumin (BSA) in Tris-buffered saline/0.1% Tween 20 (TBS-T) for 1 hr at room temperature.

ICC: 1x PBS + 5% Normal goat serum + 0.3% Triton-X100) for 1 hr at room temperature.

WB: 1:2,000 dilution in 5% BSA in TBS-T (20 mM Tris, 150 mM NaCl, 0.05% Tween 20) overnight at 4°C.

ICC: 1:200 dilution in 1xPBS + 1% BSA + 0,3% Triton-X overnight at 4°C.

WB: 1:15,000 dilution anti-mouse IgG(H+L) DyLight 800 conjugate (Cell Signaling Technologies, #5257).

ICC: for 1 hr at room temperature.

WB: Odyssey CLx Imager (LICOR Biosciences).

The same clone (C5) is sold as Novus Biologicals NB110-10872; OriGene AM05308PU-N; MilliporeSigma M6065; Santa Cruz Biotechnology sc-56725; Abnova MAB1955; Abcam ab12039; Exalpha Biologicals X1405M.