This is a knockout-validated antibody summary, based on the publication "Drp1-dependent mitochondrial fission via MiD49/51 is essential for apoptotic cristae remodeling", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot of MiD49 in mitochondrial fractions from the indicated KO HeLa cell lines. Mfn2 serves as loading control. From [1].

Rabbit polyclonal IgG

Company: Proteintech

Antibody: MiD49

Catalog number: 16413-1-AP

Summary: Rabbit polyclonal IgG against SMCR7/MID49 fusion protein. Reacts with human and mouse. Suitable for western blot, immunofluorescence, immunohistochemistry and ELISA.

Western blot

WT and MiD49-KO HeLa cells lines.

PBS containing 0.3% nonfat dry milk and 0.1% Tween 20.

AP-conjugated secondary antibodies.

Immun-Star AP Western blotting detection reagents (Bio-Rad Laboratories) and LAS-1000 (FUJIFILM).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.