Mesothelin antibody | knockout validation | Thermo Fisher MA5-16378

This is a knockout-validated antibody summary, based on publications "Regulation of invasion and peritoneal dissemination of ovarian cancer by mesothelin manipulation", as cited below [1], and "Mesothelin Expression Is Not Associated with the Presence of Cancer Stem Cell Markers SOX2 and ALDH1 in Ovarian Cancer" for immunohistochemistry knockout validation (figure 6a, 6b) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mesothelin antibody | knockout validation | Thermo Fisher MA5-16378 figure 1
Figure 1. Representative immunocytochemistry images for MSLN of OVCAR8 cell colonies obtained from soft agar assays. Scale bar 100 µm. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Thermo Fisher

Antibody: Mesothelin

Catalog number: MA5-16378

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to C-terminus of human mesothelin protein.

Reacts with human. Suitable for western blot and immunohistochemistry (paraffin).

Validation Method

Immunocytochemistry

Sample

Parental control and MSLN-KO OVCAR8 cell lines.

Primary incubation

1:50 dilution for 1 hour at room temperature.

Secondary incubation

Secondary antibody with HRP polymer (Dako REAL™ EnVision™ Detection System).

Detection

Incubation with diaminobenzidine according to the manufacturer's instructions.

References
  1. Coelho R, Ricardo S, Amaral A, Huang Y, Nunes M, Neves J, et al. Regulation of invasion and peritoneal dissemination of ovarian cancer by mesothelin manipulation. Oncogenesis. 2020;9:61 pubmed publisher
  2. Nunes M, Pacheco F, Coelho R, Leit xe3 o D, Ricardo S, David L. Mesothelin Expression Is Not Associated with the Presence of Cancer Stem Cell Markers SOX2 and ALDH1 in Ovarian Cancer. Int J Mol Sci. 2022;23: pubmed publisher