This is a knockout-validated antibody summary, based on the publication "Regulation of invasion and peritoneal dissemination of ovarian cancer by mesothelin manipulation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Representative Western blot shows loss of MSLN expression in OVCAR8 ΔMSLN clones and re-expression of EGFP and MSLN in ΔMSLN OVCAR8 cells after rescue/overexpression (rescue). From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Mesothelin

Catalog number: 65367

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues surrounding Gly557 of human mesothelin protein. Reacts with human. Suitable for western blot and immunoprecipitation.

Western blot

Parental control and MSLN-KO OVCAR8 cell lines.

5% (w/v) BSA (Sigma- Aldrich) or non-fat milk in TBS-T.

1:1,000 dilution in 5% (w/v) BSA or non-fat milk in TBST at 4°C overnight.

1:10,000 dilution HRP-conjugated anti-rabbit secondary antibodies (Cell Signaling) in TBS-T for 3 hours at room temperature.

Super Signal West Dura Extended Substrate (Thermo Fisher Scientific).