Merlin antibody | knockout validation | Cell Signaling 6995

This is a knockout-validated antibody summary, based on the publication "Genome-wide CRISPR screen identifies noncanonical NF-κB signaling as a regulator of density-dependent proliferation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Merlin antibody | knockout validation | Cell Signaling 6995 figure 1
Figure 1. Immunoblots of control and NF2 KO EpH4 cells. α-tubulin was used as a loading control. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Merlin

Catalog number: 6995

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues near the amino terminus of human Merlin protein. Reacts with human, mouse and rat. Suitable for Western blot and immunoprecipitation.

Validation Method

Western blot

Sample

Control and NF2 KO cells.

Primary incubation

1:750 dilution.

References
  1. Fomicheva M, Macara I. Genome-wide CRISPR screen identifies noncanonical NF-κB signaling as a regulator of density-dependent proliferation. elife. 2020;9: pubmed publisher