Meis1 antibody | knockout validation | Santa Cruz sc-10599
DOI
//dx.doi.org/10.13070/ko.en.6.1636
Date
2016-09-24

This is a knockout-validated antibody summary, based on the publication "Loss of the transcription factor Meis1 prevents sympathetic neurons target-field innervation and increases susceptibility to sudden cardiac death", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Meis1 antibody | knockout validation | Santa Cruz sc-10599 figure 1
Figure 1. Immunochemistry for Meis1 and the transcription factor Phox2b on sections of superior cervical ganglias from E16.5 WT and PLATCRE::Meis1LoxP/LoxP mice from figure 1 [1].
Antibody information

Antibody: Meis1

Catalog number: sc-10599

Summary: Goat polyclonal IgG against an epitope mapping at the C-terminus of Meis1 of human origin. Recommended for detection of Meis1 and Meis2 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA. Also reactive with additional species, including equine, bovine and avian.

Company: Santa Cruz

Validation Method

IHC-F, immunohistochemistry - frozen sections.

Sample

Sections of superior cervical ganglia from E16.5 WT and PLATCRE::Meis1LoxP/LoxP mice. An epitope retrieval step: immersion of the sections for 15 min at 68° in sodium citrate buffer (10 mM Sodium Citrate, 0.05% Tween 20 at pH 6.

Blocking agent

1 h in PBS, 0.2% BSA, 0.1% Triton X-100 and 4% donkey serum.

Primary incubation

dilution at 1/100

Detection

confocal microscopy (Leica SP5-SMD, Germany)

References
  1. Bouilloux F, Thireau J, Ventéo S, Farah C, Karam S, Dauvilliers Y, et al. Loss of the transcription factor Meis1 prevents sympathetic neurons target-field innervation and increases susceptibility to sudden cardiac death. elife. 2016;5: pubmed publisher