This is a knockout-validated antibody summary, based on the publication "Loss of the transcription factor Meis1 prevents sympathetic neurons target-field innervation and increases susceptibility to sudden cardiac death", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot analysis for Meis1 from adult stellate ganglia from WT and PLATCRE::Meis1LoxP/LoxP mice. Beta-tubulin is used as loading control from figure [1].

Antibody: Meis1

Catalog number: 05-779

Summary: Mouse monoclonal antibody detecting Meis 1/2/3 of human and mouse origin. Used for immunoprecipitation, western blot, and immunohistochemistry.

Company: Millipore, now MilliporeSigma

Western blot

Adult stellate ganglia. Cells were lysed in Laemmli buffer containing 62.5 mM Tris-HCl, pH 6.8, 25% glycerol, 2% SDS, 0.01% Bromophenol Blue and 5% β-mercaptoethanol.

dilution at 1/100

HRP-conjugated goat anti-mouse secondary antibody (Jackson ImmunoResearch, 115-035-174, 1/500, Baltimore, PA, USA)

chemo-luminescence using the Pierce ECL Plus Western Blotting Substrate (Thermo Scientific, 32132, Waltham, MA, USA). Signals were detected using autoradiographic films (Sigma Aldrich, Z363022-50EA, Saint-Louis, Missouri, USA) or light sensitive camera (BioRad, ChemiDo XRS + System, Hercules, California, USA).