Mcl-1 antibody | knockout validation | Cell Signaling 94296

This is a knockout-validated antibody summary, based on the publication "Breast cancer dependence on MCL-1 is due to its canonical anti-apoptotic function", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mcl-1 antibody | knockout validation | Cell Signaling 94296 figure 1
Figure 1. Western blot showing impact of CRE activation on MCL-1 and BCL-XL expression in a cell line from MMTV-PyMT;RosaCRE-ERT2;Mcl1fl/fl tumour. CRE activated in vitro with 100nM 4-hydroxytamoxifen (4-OHT), samples harvested after 72h. MCL-1 is efficiently deleted, BCL-XL level is not increased. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Mcl-1

Catalog number: 94296

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues surrounding Pro60 of mouse Mcl-1 protein. Reacts with human, mouse, and rat. Suitable for western blot, immunoprecipitation, immunofluorescence/immunocytochemistry and flow cytometry (fixed/permeabilized).

Validation Method

Western blot

Sample

Control and MCL-1-KO MMTV-PyMT mice mammary tumor cells.

Secondary incubation

IRDye 800CW Donkey Anti-Rabbit IgG

Detection

Li-cor Odyssey CLx.

References
  1. Campbell K, Mason S, Winder M, Willemsen R, Cloix C, Lawson H, et al. Breast cancer dependence on MCL-1 is due to its canonical anti-apoptotic function. Cell Death Differ. 2021;: pubmed publisher