This is a knockout-validated antibody summary, based on the publication "53BP1-RIF1-shieldin counteracts DSB resection through CST- and Polα-dependent fill-in", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Abcam
Antibody: Mad2L2/REV7
Catalog number: ab180579
Summary: Rabbit monoclonal IgG against a synthetic peptide within human Mad2L2/REV7(aa 150 to the C-terminus). Reacts with mouse, rat and human. Suitable for western blot, immunoprecipitation, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin) and flow cytometry.
Western blot
Control and Rev7-KO MEF cells.
Immunoblots showing loss of Rev7 and Stn1 in the indicated TPP1F/F Rev7+/+ MEFs and TPP1F/F Rev7−/− (CRISPR) clones treated with Cre (96 h) and/or Stn1 shRNA as indicated. Please see Figure 1b in the article [1].
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