This is a knockout-validated antibody summary, based on the publication "Triggering MSR1 promotes JNK-mediated inflammation in IL-4-activated macrophages", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

[enlarge]

Figure 1. Msr1 +/+ (WT) and Msr1 −/− (KO) M0 macrophages and M2 (IL‐4) macrophages untreated or stimulated with the MSR1 ligands fucoidan or oxLDL (50 μg/ml, 30 min) were analysed for the indicated proteins. ITGAM (CD11b) serves as a loading control. From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: Macrophage Scavenger Receptor I/SR-A

Catalog number: ab151707

Summary: Rabbit monoclonal IgG against a synthetic peptide within human Macrophage Scavenger Receptor I/SR-A (MSR1, aa 1-100, N terminal). Reacts with mouse, rat, and human. Suitable for western blot. Unsuitable for immunocytochemistry, immunohistochemistry (paraffin) or immunoprecipitation.

Western blot

Wild‐type (WT) and MSR1 KO bone marrow‐derived macrophages from mice. Cells or phagosomes were lysed directly in 2× Laemmli buffer.

5% (w/v) skim milk in TBS containing 0.1% (v/v) Tween 20 for 1 h.

Overnight.

HRP‐labelled secondary antibodies.

ECL and X‐ray films.