This is a knockout-validated antibody summary, based on the publication "Dual role of MUC1 mucin in kidney ischemia-reperfusion injury: Nephroprotector in early phase, but pro-fibrotic in late phase", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Armenian hamster monoclonal IgG

Company: ThermoFisher

Antibody: MUC1

Catalog number: MA5-11202

Summary: Armenian hamster monoclonal IgG against a synthetic peptide corresponding to aa 239-255 from the cytoplasmic tail of MUC1. Reacts with human, mouse and rat. Suitable for western blot, immunofluorescence/immunocytochemistry, immunohistochemistry (frozen and paraffin), immunoprecipitation, ChIP and flow cytometry.

Western blot | Immunohistochemistry

Kidney of WT and Muc1 KO mice. WB: Whole-kidney extracts were dissociated with the gentleMACS™ Dissociator (Miltenyi Biotec) and homogenized in cold phosphate-buffered saline with protease inhibitors (aprotinin, leupeptin, pepstatin A). IHC: Paraffin-embedded renal sections.

IHC: overnight at +4°C.

Representative western blots analysis with anti-MUC1 (CT2 clone) and anti-GAPDH antibodies from lysates obtained from whole-kidney of WT and Muc1 KO mice non–operated (sham) and subject to IR (n=2). Immunohistochemistry study performed with anti-MUC1 antibody (CT2). Representative sections showing increased cytoplasmic tubular Muc1 expression in IR-treated kidneys of WT mice. IR, ischemia-reperfusion; Sham, sham operated; WT, wild type. Please see Figure 2b,d in the article [1].