This is a knockout-validated antibody summary, based on the publication "Downregulation of MTSS1 in acute myeloid leukemia is associated with a poor prognosis, chemotherapy resistance, and disease aggressiveness", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Knock-out of MTSS1 in single cell clones of sgRNA-transduced TF-1 cells was confirmed by immunoblot analysis; GAPDH was used as loading control. C1-C4, clone numbers. The antibody recognizes an epitope close to the C-terminus of the protein that is encoded by all reported transcript variants. From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: MTSS1

Catalog number: 93065

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues surrounding Val650 of human MTSS1 protein. Reacts with human and rat. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin), and immunocytochemistry/immunofluorescence.

Western blot

Control and MTSS1-KO TF-1 cells.

5% non-fat dry milk (AppliChem, Darmstadt, Germany)/TBS-T (40 mM Tris/HCl pH 7.6, 273 mM NaCl, 0.1% Tween 20 (Sigma-Aldrich)) 1 h at room temperature.

1:2,000 dilution in blocking buffer overnight at 4 °C.

In blocking buffer for 1 h at room temperature.

SuperSignal West Femto or Pico Chemiluminescent Substrates (Thermo Fisher Scientific), and signals were detected and quantified on a ChemiDoc Touch Imaging System (Bio-Rad).