MTHFD2 antibody | knockout validation | Cell Signaling 41377

This is a knockout-validated antibody summary, based on the publication "The folate cycle enzyme MTHFD2 induces cancer immune evasion through PD-L1 up-regulation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

MTHFD2 antibody | knockout validation | Cell Signaling 41377 figure 1
Figure 1. SW1990 cells stably transduced with indicated sgRNAs by lentivirus, immunoblotting analyses were performed using the indicated antibodies. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: MTHFD2

Catalog number: 41377

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues surrounding Leu340 of human MTHFD2 protein. Reacts with human. Suitable for western blot and immunoprecipitation.

Validation Method

Western blot

Sample

Control and MTHFD2-KO SW1990 cells.

Primary incubation

1:1,1000 dilution.

Detection

Chemiluminescence imaging system (Tanon).

References
  1. Shang M, Yang H, Yang R, Chen T, Fu Y, Li Y, et al. The folate cycle enzyme MTHFD2 induces cancer immune evasion through PD-L1 up-regulation. Nat Commun. 2021;12:1940 pubmed publisher