This is a knockout-validated antibody summary, based on the publication "Metadherin enhances vulnerability of cancer cells to ferroptosis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. WB: Using MDA-MB-231 cells, knocking out MTDH increased protein levels of SLC3A2 and GPx4, which were reversed by re-expression of MTDH. From [1].

Company: Santa Cruz

Antibody: MTDH

Catalog number: sc-517220

Summary: Mouse monoclonal IgG1 against a recombinant protein corresponding to amino acids 378-579 of human MTDH. Recommended for detection of MTDH of human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin), flow cytometry and ELISA.

Figure 2. IHC: MTDH WT and KO MDA-MB-231 cells were implanted into NSG mice (four mice each group), and tumors were harvested to check expression levels of SLC3A2 and GPx4. CT: control using WT cells. From [1].

Western blot | Immunohistochemistry

Control and MTDH-KO MDA-MB-231 cells and tumors.

WB: 5% non-fat milk.

WB: 1:250 dilution overnight at 4 °C.

WB: 1:10,000 dilution secondary antibody (7076S, Cell Signaling Technology) at room temperature for 2 h.

WB: Bio-Rad ChemiDoc system.