This is a knockout-validated antibody summary, based on the publication "MST4 kinase suppresses gastric tumorigenesis by limiting YAP activation via a non-canonical pathway", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG1 κ
Company: Santa Cruz
Antibody: MST-4
Catalog number: sc-376649
Summary: Mouse monoclonal IgG1 κ specific for an epitope mapping between amino acids 307-339 near the C-terminus of human MST-4. Recommended for detection of MST-4 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA. Also reactive with additional species, including and canine, bovine and porcine.
Western blot
WT and MST4 KO 293A cells.
1:1,000 dilution.
1:4,000 dilution HRP goat anti-mouse (Thermo Fisher Scientific, 31430).
Mini Chemiluminescent Imaging and Analysis System (Beijing Sage Creation Science Co., Ltd.).
293A cells (WT and MST4 KO) were treated with serum-free medium for 12 h. Fractionation assay was performed to examine YAP protein distribution via western blotting analysis using indicated antibodies. Short, short exposure; Long, long exposure. Please see Figure 1f in the article [1].
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