This is a knockout-validated antibody summary, based on the publication "Image-based detection and targeting of therapy resistance in pancreatic adenocarcinoma", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Abcam
Antibody: MSI2
Catalog number: ab76148
Summary: Rabbit monoclonal IgG against a synthetic peptide derived from human MSI2. Reacts with human, mouse, and rat. Suitable for western blot, immunohistochemistry (paraffin), immunocytochemistry/immunofluorescence and flow cytometry. Unsuitable for immunoprecipitation.
Immunohistochemistry
WT and Ms2 knockout KPf/fC mice. Pancreatic cancer tissues were fixed in 4% paraformaldehyde and paraffin embedded.
TBS or PBS containing 0.1% Triton X100 (Sigma Aldrich), 10% goat or donkey serum (Sigma Aldrich), and 5% bovine serum albumin and 3% H2O2 for 15 minutes.
1:500 dilution.
Biotinylated secondary antibodies (Vector Laboratories) was performed for 45 minutes at room temperature.
ImmPACT NovaRED Kit (Vector Laboratories) was used per manufacturer's protocol.
Immunohistochemical staining for (a) IgG control (n=4), or (b-c, red) Msi2 in 13 week old WT-KPf/fC (n=4) and Msi2−/−KPf/fC (n=4) mice. Please see Extended Data Figure 5 in the article [1].
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