This is a knockout-validated antibody summary, based on the publication "Musashi proteins are post-transcriptional regulators of the epithelial-luminal cell state", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot analysis of Musashi 1/2 conditional double knockout in neural stem cells, exposed to 4-OHT for 72 hr. Actin is used as a loading control. From [1].

Antibody: MSI1/2

Catalog number: 2154

Summary: Rabbit polyclonal raised against a synthetic peptide corresponding to residues of human Musashi. Detects endogenous levels of total Musashi 1 and 2 protein from human, mouse, and rat, and is predicted to react with zebrafish (based on 100% sequence homology). Recommended to use in western blotting and immunofluorescence (frozen).

Company: Cell Signaling Technology

Western blot

Musashi knockout in neural stem cells

Dilution 1:800

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.