MSH6 antibody | knockout validation | Cell Signaling 5424

This is a knockout-validated antibody summary, based on the publication "Validating the concept of mutational signatures with isogenic cell models", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

MSH6 antibody | knockout validation | Cell Signaling 5424 figure 1
Figure 1. Immunoblots for expression of the indicated proteins in CRISPR-Cas9 edited human HAP1 clones. Actin serves as a loading control. “*” denotes a non-specific band. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: MSH6

Catalog number: 5424

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues near the carboxy terminus of human MSH6. Reacts with human and monkey. Suitable for western blot and immunofluorescence/immunocytochemistry.

Validation Method

Western blot

Sample

Control and MSH6 human HAP1 cells. Cell extracts were prepared using RIPA lysis buffer (NEB) with protease (Sigma) and phosphatase (Sigma) inhibitors.

Primary incubation

1:1,000 dilution.

References
  1. Zou X, Owusu M, Harris R, Jackson S, Loizou J, Nik Zainal S. Validating the concept of mutational signatures with isogenic cell models. Nat Commun. 2018;9:1744 pubmed publisher