This is a knockout-validated antibody summary, based on the publication "ARTICLE TITLE", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. From Figure 4 in [1]. Western blot of lysates of testes, brain, liver and ovary of wild-type mice and mice deleted for MSH2, MSH3 or MSH6. Blots were probed with anti-MSH6 antibody. Laminin B1 and actin serve as a loading controls.

Mouse monoclonal

Company: BD Biosciences

Antibody: MSH6

Catalog number: 610918

Summary: Mouse monoclonal antibody raised against residues 225-333 of human MSH6.

Supplier recommended for WB and IF.

Reacts with human, mouse, rat and dog MSH6.

Western blot

Lysates of testes, brain, liver and ovary of wild-type mice and mice deleted for MSH2, MSH3 or MSH6.

TBST containing 5% ECL Prime blocking agent (GE Healthcare).

Anti-MSH6 antibody diluted 1:1000.

Anti-mouse IgG diluted 1:5000 (NA931V; GE Healthcare).

ECL Prime detection reagent (GE Healthcare).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.